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custom total internal reflection fluorescence tirf microscope  (Nikon)


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    Nikon custom total internal reflection fluorescence tirf microscope
    Custom Total Internal Reflection Fluorescence Tirf Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 60052 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/internal+reflection+fluorescence+microscope/Objectives/bio_rxiv__64898__2026__01__23__700875-252-23-30
    Average 99 stars, based on 60052 article reviews
    custom total internal reflection fluorescence tirf microscope - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Protonation-Regulated Membrane-Insertion Dynamics of pH Low-Insertion Peptide: Metastable Molecular Conformations and Their Transitions.
    Article Snippet: .. To observe the fluorescence of TAMRApHLIP, we employed an objective-based total internal reflection fluorescence microscope (TIRFM; Ti2, Nikon) equipped with an EMCCD camera (DU-897, Andor). ..

    Article Title: Absence of Shb impairs insulin secretion by elevated FAK activity in pancreatic islets
    Article Snippet: .. The islets were then placed on a poly-L-lysine-coated coverslip in the superfusion chamber on the thermostated stage of a total internal reflection fluorescence microscope (Eclipse Ti with a 60!, 1.45-NA objective, Nikon, Japan). .. A diode-pumped solid-state laser (Cobolt AB, Solna, Sweden) provided 491 nm excitation light and the emission was selected by a 527/27-nm (center wavelength/half-bandwidth) interference filter (Semrock, Rochester, NY, USA) and recorded by an EMCCD camera (DU-897; Andor, Belfast, UK) controlled by MetaFluor software (Molecular Devices, Sunnyvale, CA, USA).

    Article Title: Structure of a fully assembled γδ T cell antigen receptor
    Article Snippet: Imaging buffer, consisting of TN buffer (50 mM Tris-HCl pH 8.0 and 10 mM NaCl), the oxygen scavenger system GLOX (0.5 mg ml −1 glucose oxidase (Sigma-Aldrich, no. G2133), 40 mg ml −1 catalase (Sigma-Aldrich, no. C-100) and 10% w/v glucose) and 10 mM 2-aminoethanethiol (Sigma-Aldrich, no. M6500), was used for single-molecule imaging with direct stochastic optical reconstruction microscopy (dSTORM). .. Imaging sequences for dSTORM were acquired on a total internal reflection fluorescence microscope (Nikon N-STORM 5.0) equipped with a ×100/1.49 numerical aperture oil-immersion objective and lasers (405, 473, 561 and 640 nm). ..

    Article Title: Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence
    Article Snippet: .. DNA-PAINT measurements were performed on an inverted total internal reflection fluorescence microscope (Nikon) with a 100×/NA 1.49 objective. ..

    Article Title: In vitro reconstitution of a highly processive recombinant human dynein complex.
    Article Snippet: .. All microscopy was performed at 25 1°C with a total internal reflection fluorescence microscope (Nikon) equipped with a 100× objective (Nikon, 1.49 NA Oil, APO TIRF). a 2014 MRC Laboratory of Molecular Biology The EMBO Journal Vol 33 | No 17 | 2014 D ow nloaded from https://w w w .em bopress.org on June 9, 2024 from IP 2401:4900:1ce3:4cca:11bb:356a:c010:b627. .. The imaging system was equipped with the following lasers: 150 mW 488 nm, 150 mW 561 nm laser (both Coherent Sapphire) and 100 mW 641 nm (Coherent Cube).

    Article Title: Spatiotemporal stop-and-go dynamics of the mitochondrial TOM core complex correlates with channel activity.
    Article Snippet: .. An inverted total internal reflection fluorescence microscope (Ti-E Nikon) was used to image DIB membranes under TIRF illumination using a 488 nm laser (Visitron). .. The supernatant containing OmpF was dialyzed overnight against 20 mM Tris, pH 8, 1 mM EDTA and 1% (w/v) n-octyl polyoxyethylene (Octyl-POE, Bachem, Switzerland).

    Article Title: Mechano-fluorescence actuation in single synaptic vesicles with a DNA framework nanomachine.
    Article Snippet: Biomimetic machines that can convert mechanical actuation to adaptive coloration in a manner analogous to cephalopods have found widespread applications at various length scales.. At the nanoscale, a transmutable nanomachine with adaptive colors that can sense and mediate cellular or intracellular interactions is highly desirable.. Here, we report the design of a DNA framework nanomachine (DFN) that can autonomously change shape in response to pH variations in single synaptic vesicles, which, in turn, displays adaptive fluorescent colors with a mechano-fluorescence actuation mechanism.

    Article Title: Protonation-Regulated Membrane-Insertion Dynamics of pH Low-Insertion Peptide: Metastable Molecular Conformations and Their Transitions
    Article Snippet: .. To observe the fluorescence of TAMRA-pHLIP, we employed an objective-based total internal reflection fluorescence microscope (TIRFM; Ti2, Nikon) equipped with an EMCCD camera (DU-897, Andor). ..

    Microscopy:

    Article Title: Protonation-Regulated Membrane-Insertion Dynamics of pH Low-Insertion Peptide: Metastable Molecular Conformations and Their Transitions.
    Article Snippet: .. To observe the fluorescence of TAMRApHLIP, we employed an objective-based total internal reflection fluorescence microscope (TIRFM; Ti2, Nikon) equipped with an EMCCD camera (DU-897, Andor). ..

    Article Title: Absence of Shb impairs insulin secretion by elevated FAK activity in pancreatic islets
    Article Snippet: .. The islets were then placed on a poly-L-lysine-coated coverslip in the superfusion chamber on the thermostated stage of a total internal reflection fluorescence microscope (Eclipse Ti with a 60!, 1.45-NA objective, Nikon, Japan). .. A diode-pumped solid-state laser (Cobolt AB, Solna, Sweden) provided 491 nm excitation light and the emission was selected by a 527/27-nm (center wavelength/half-bandwidth) interference filter (Semrock, Rochester, NY, USA) and recorded by an EMCCD camera (DU-897; Andor, Belfast, UK) controlled by MetaFluor software (Molecular Devices, Sunnyvale, CA, USA).

    Article Title: Structure of a fully assembled γδ T cell antigen receptor
    Article Snippet: Imaging buffer, consisting of TN buffer (50 mM Tris-HCl pH 8.0 and 10 mM NaCl), the oxygen scavenger system GLOX (0.5 mg ml −1 glucose oxidase (Sigma-Aldrich, no. G2133), 40 mg ml −1 catalase (Sigma-Aldrich, no. C-100) and 10% w/v glucose) and 10 mM 2-aminoethanethiol (Sigma-Aldrich, no. M6500), was used for single-molecule imaging with direct stochastic optical reconstruction microscopy (dSTORM). .. Imaging sequences for dSTORM were acquired on a total internal reflection fluorescence microscope (Nikon N-STORM 5.0) equipped with a ×100/1.49 numerical aperture oil-immersion objective and lasers (405, 473, 561 and 640 nm). ..

    Article Title: Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence
    Article Snippet: .. DNA-PAINT measurements were performed on an inverted total internal reflection fluorescence microscope (Nikon) with a 100×/NA 1.49 objective. ..

    Article Title: In vitro reconstitution of a highly processive recombinant human dynein complex.
    Article Snippet: .. All microscopy was performed at 25 1°C with a total internal reflection fluorescence microscope (Nikon) equipped with a 100× objective (Nikon, 1.49 NA Oil, APO TIRF). a 2014 MRC Laboratory of Molecular Biology The EMBO Journal Vol 33 | No 17 | 2014 D ow nloaded from https://w w w .em bopress.org on June 9, 2024 from IP 2401:4900:1ce3:4cca:11bb:356a:c010:b627. .. The imaging system was equipped with the following lasers: 150 mW 488 nm, 150 mW 561 nm laser (both Coherent Sapphire) and 100 mW 641 nm (Coherent Cube).

    Article Title: Spatiotemporal stop-and-go dynamics of the mitochondrial TOM core complex correlates with channel activity.
    Article Snippet: .. An inverted total internal reflection fluorescence microscope (Ti-E Nikon) was used to image DIB membranes under TIRF illumination using a 488 nm laser (Visitron). .. The supernatant containing OmpF was dialyzed overnight against 20 mM Tris, pH 8, 1 mM EDTA and 1% (w/v) n-octyl polyoxyethylene (Octyl-POE, Bachem, Switzerland).

    Article Title: Mechano-fluorescence actuation in single synaptic vesicles with a DNA framework nanomachine.
    Article Snippet: Biomimetic machines that can convert mechanical actuation to adaptive coloration in a manner analogous to cephalopods have found widespread applications at various length scales.. At the nanoscale, a transmutable nanomachine with adaptive colors that can sense and mediate cellular or intracellular interactions is highly desirable.. Here, we report the design of a DNA framework nanomachine (DFN) that can autonomously change shape in response to pH variations in single synaptic vesicles, which, in turn, displays adaptive fluorescent colors with a mechano-fluorescence actuation mechanism.

    Article Title: Protonation-Regulated Membrane-Insertion Dynamics of pH Low-Insertion Peptide: Metastable Molecular Conformations and Their Transitions
    Article Snippet: .. To observe the fluorescence of TAMRA-pHLIP, we employed an objective-based total internal reflection fluorescence microscope (TIRFM; Ti2, Nikon) equipped with an EMCCD camera (DU-897, Andor). ..

    Imaging:

    Article Title: Structure of a fully assembled γδ T cell antigen receptor
    Article Snippet: Imaging buffer, consisting of TN buffer (50 mM Tris-HCl pH 8.0 and 10 mM NaCl), the oxygen scavenger system GLOX (0.5 mg ml −1 glucose oxidase (Sigma-Aldrich, no. G2133), 40 mg ml −1 catalase (Sigma-Aldrich, no. C-100) and 10% w/v glucose) and 10 mM 2-aminoethanethiol (Sigma-Aldrich, no. M6500), was used for single-molecule imaging with direct stochastic optical reconstruction microscopy (dSTORM). .. Imaging sequences for dSTORM were acquired on a total internal reflection fluorescence microscope (Nikon N-STORM 5.0) equipped with a ×100/1.49 numerical aperture oil-immersion objective and lasers (405, 473, 561 and 640 nm). ..



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    a Time-lapse <t>TIRF</t> microscopy images of debranching events in the absence (left) or presence (right) of Coro7 FL. The flow rate is 200 µL min − 1 , corresponding to an average force of ~0.45 pN on an average ~2 µm-long branch . b Time courses of branch dissociation in the absence or presence of Coro7 FL. Solid lines through the data represent best fits to single (without Coro7 FL, black) or double (with Coro7 FL, blue shades) exponentials. c Debranching rate constants as a function of Coro7 FL concentration. The individual data points represent the observed debranching rate constants obtained from the best fits of the time courses shown in ( b ) to double exponentials. The teal and cyan horizontal lines represent the best global fits of the debranching time courses shown in ( b ) to a double exponential with a single rate constant for each phase (fast and slow). d Dependence of the fast-debranching phase amplitudes on Coro7 FL concentration. The blue circles represent the amplitudes obtained from individual fitting of the time courses shown in ( b ) to double exponentials, and the red circles represent the relative amplitudes obtained from global fitting. The solid line through the data represents the best fit to a rectangular hyperbola, yielding an apparent Coro7 FL binding affinity of 31 ± 1 nM for both individually and globally fitted parameters. e – g Time courses of branch dissociation in the absence or presence of various concentrations of Coro7 construct β2CA (solid lines indicate double-exponential fits) and constructs β1β2 and CA (solid lines indicate single-exponential fits). The graphs in ( b , e – g ) show averages; each condition was repeated two to four times, with an average of 43 fields of view, 113 mother filaments, and 155 branches analyzed per condition. In ( c and d ), the error bars represent standard deviation (SD) of the best-fit parameters from the time courses shown in ( b ), as calculated using Origin software. Source data are provided in the Source Data file.
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    a Time-lapse <t>TIRF</t> microscopy images of debranching events in the absence (left) or presence (right) of Coro7 FL. The flow rate is 200 µL min − 1 , corresponding to an average force of ~0.45 pN on an average ~2 µm-long branch . b Time courses of branch dissociation in the absence or presence of Coro7 FL. Solid lines through the data represent best fits to single (without Coro7 FL, black) or double (with Coro7 FL, blue shades) exponentials. c Debranching rate constants as a function of Coro7 FL concentration. The individual data points represent the observed debranching rate constants obtained from the best fits of the time courses shown in ( b ) to double exponentials. The teal and cyan horizontal lines represent the best global fits of the debranching time courses shown in ( b ) to a double exponential with a single rate constant for each phase (fast and slow). d Dependence of the fast-debranching phase amplitudes on Coro7 FL concentration. The blue circles represent the amplitudes obtained from individual fitting of the time courses shown in ( b ) to double exponentials, and the red circles represent the relative amplitudes obtained from global fitting. The solid line through the data represents the best fit to a rectangular hyperbola, yielding an apparent Coro7 FL binding affinity of 31 ± 1 nM for both individually and globally fitted parameters. e – g Time courses of branch dissociation in the absence or presence of various concentrations of Coro7 construct β2CA (solid lines indicate double-exponential fits) and constructs β1β2 and CA (solid lines indicate single-exponential fits). The graphs in ( b , e – g ) show averages; each condition was repeated two to four times, with an average of 43 fields of view, 113 mother filaments, and 155 branches analyzed per condition. In ( c and d ), the error bars represent standard deviation (SD) of the best-fit parameters from the time courses shown in ( b ), as calculated using Origin software. Source data are provided in the Source Data file.
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    Image Search Results


    DNA-PAINT experiment on single gold nanospheres. (a) Schematic of a gold nanosphere sample on a glass substrate excited with an epi-illumination fluorescence microscope. Fluorescent intensity time traces are recorded at 10 frames per second, showing bursts with an event typically lasting for 10 frames. Each fluorescent event, averaged over all frames above the threshold, provides a polarized PSF image. Four example events with the extracted α and DoLP indicate single-emitter binding to a particle at different locations. Scale bar of 200 nm applies to all PSFs. (b) Scatter plot of DoLP and α apparent for fluorescence events on six single nanospheres. (c) DoLP as a function of integrated fluorescence intensity showing that brighter events exhibit a higher DoLP because they occur at the equator of the particle.

    Journal: Nanophotonics

    Article Title: Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence

    doi: 10.1515/nanoph-2025-0352

    Figure Lengend Snippet: DNA-PAINT experiment on single gold nanospheres. (a) Schematic of a gold nanosphere sample on a glass substrate excited with an epi-illumination fluorescence microscope. Fluorescent intensity time traces are recorded at 10 frames per second, showing bursts with an event typically lasting for 10 frames. Each fluorescent event, averaged over all frames above the threshold, provides a polarized PSF image. Four example events with the extracted α and DoLP indicate single-emitter binding to a particle at different locations. Scale bar of 200 nm applies to all PSFs. (b) Scatter plot of DoLP and α apparent for fluorescence events on six single nanospheres. (c) DoLP as a function of integrated fluorescence intensity showing that brighter events exhibit a higher DoLP because they occur at the equator of the particle.

    Article Snippet: DNA-PAINT measurements were performed on an inverted total internal reflection fluorescence microscope (Nikon) with a 100×/NA 1.49 objective.

    Techniques: Fluorescence, Microscopy, Binding Assay

    a Time-lapse TIRF microscopy images of debranching events in the absence (left) or presence (right) of Coro7 FL. The flow rate is 200 µL min − 1 , corresponding to an average force of ~0.45 pN on an average ~2 µm-long branch . b Time courses of branch dissociation in the absence or presence of Coro7 FL. Solid lines through the data represent best fits to single (without Coro7 FL, black) or double (with Coro7 FL, blue shades) exponentials. c Debranching rate constants as a function of Coro7 FL concentration. The individual data points represent the observed debranching rate constants obtained from the best fits of the time courses shown in ( b ) to double exponentials. The teal and cyan horizontal lines represent the best global fits of the debranching time courses shown in ( b ) to a double exponential with a single rate constant for each phase (fast and slow). d Dependence of the fast-debranching phase amplitudes on Coro7 FL concentration. The blue circles represent the amplitudes obtained from individual fitting of the time courses shown in ( b ) to double exponentials, and the red circles represent the relative amplitudes obtained from global fitting. The solid line through the data represents the best fit to a rectangular hyperbola, yielding an apparent Coro7 FL binding affinity of 31 ± 1 nM for both individually and globally fitted parameters. e – g Time courses of branch dissociation in the absence or presence of various concentrations of Coro7 construct β2CA (solid lines indicate double-exponential fits) and constructs β1β2 and CA (solid lines indicate single-exponential fits). The graphs in ( b , e – g ) show averages; each condition was repeated two to four times, with an average of 43 fields of view, 113 mother filaments, and 155 branches analyzed per condition. In ( c and d ), the error bars represent standard deviation (SD) of the best-fit parameters from the time courses shown in ( b ), as calculated using Origin software. Source data are provided in the Source Data file.

    Journal: Nature Communications

    Article Title: Mechanism of Arp2/3 complex branch disassembly by human Coro7

    doi: 10.1038/s41467-025-64787-z

    Figure Lengend Snippet: a Time-lapse TIRF microscopy images of debranching events in the absence (left) or presence (right) of Coro7 FL. The flow rate is 200 µL min − 1 , corresponding to an average force of ~0.45 pN on an average ~2 µm-long branch . b Time courses of branch dissociation in the absence or presence of Coro7 FL. Solid lines through the data represent best fits to single (without Coro7 FL, black) or double (with Coro7 FL, blue shades) exponentials. c Debranching rate constants as a function of Coro7 FL concentration. The individual data points represent the observed debranching rate constants obtained from the best fits of the time courses shown in ( b ) to double exponentials. The teal and cyan horizontal lines represent the best global fits of the debranching time courses shown in ( b ) to a double exponential with a single rate constant for each phase (fast and slow). d Dependence of the fast-debranching phase amplitudes on Coro7 FL concentration. The blue circles represent the amplitudes obtained from individual fitting of the time courses shown in ( b ) to double exponentials, and the red circles represent the relative amplitudes obtained from global fitting. The solid line through the data represents the best fit to a rectangular hyperbola, yielding an apparent Coro7 FL binding affinity of 31 ± 1 nM for both individually and globally fitted parameters. e – g Time courses of branch dissociation in the absence or presence of various concentrations of Coro7 construct β2CA (solid lines indicate double-exponential fits) and constructs β1β2 and CA (solid lines indicate single-exponential fits). The graphs in ( b , e – g ) show averages; each condition was repeated two to four times, with an average of 43 fields of view, 113 mother filaments, and 155 branches analyzed per condition. In ( c and d ), the error bars represent standard deviation (SD) of the best-fit parameters from the time courses shown in ( b ), as calculated using Origin software. Source data are provided in the Source Data file.

    Article Snippet: Direct visualization of actin filaments and branches was performed using a Nikon Eclipse Ti2 total internal reflection fluorescence (TIRF) microscope equipped with an SR HP Apo TIRF ×100 objective (Nikon) and an Andor iXon897 EMCCD camera.

    Techniques: Microscopy, Concentration Assay, Binding Assay, Construct, Standard Deviation, Software